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SCIENCE - Le LOV à l'honneur

La revue SCIENCE publie un article de revue de Jean-Pierre Gattuso et ses collaborateurs intitulé "Contrasting futures for ocean andsociety from different anthropogenic". Numéro du 3 juillet 2015 • VOL 349 ISSUE 6243Nouvelle fenêtre

 

The ocean moderates anthropogenic climate change at the cost of profound alterations ...

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PhytoPULSE : PhytoPlankton cULturing SystEm

PrincipLe

PhytoPULSE is a platform made up of various automated photobioreactors allowing the culturing of microalgae over long durations and the fine control of their growth conditions. All the culture devices and associated controllers make it possible to connect the photobioreactors to the electronic environment necessary for the automation of the system. The PhytoPULSE allows to control up to 8 continuous cultures simultaneously for several months. The studies conducted are designed to understand the physiology of phytoplankton under specific growth conditions (natural or not), to make varietal selection of microalgae strains, to optimize culture conditions and to provide measurements of a quality that allows mathematical models validation. It also makes it possible to explore possible industrial uses for marine microalgae (biorefinery, production of metabolites of interest)

 

SEMPO2

ContrOL

The parameters controlled by the PhytoPULSE that determine the growing conditions are :

  • Light: the light intensity can be either continuous or variable, such as a day/night cycle, or it can simulate the light received by a moving particle in an oceanic  water column or in a dense microalgal culture. The new version of the system allows the spectral composition of the incident light to be modulated using a wide range of LEDs.
  • Temperature: Thermostatic water circulates in the double wall of the photobioreactors using programmable cryostats (daytime variations).
  • Growth rate: the dilution rate, adjusted by high precision pumps, allows the algae growth rate to be controlled. The cultures can thus be operated in batch mode as well as in various continuous modes (chemostat and turbidostat). For the turbidostat mode, the pumps that control the supply of fresh medium to the cultures are controlled by the turbidity measured in the photobioreactors.
  • Limiting factor: it is possible to enrich cultures with nutrients according to time-varying regimes, continuous or pulsed. The device also allows a limitation by light.
  • pH: the pH is controlled to a set value by bubbling CO2-enriched air.

 

The culturing system is coupled to several devices that have been automated in the laboratory. This technology allows direct sampling from photobioreactors and acquisition of real-time, high-frequency data. The parameters that can be measured automatically are:

  • Optical density of cultures
  • Number and size distribution of cells thanks to a particle counter (HIAC) associated with an automated dilutor (acquisition frequency: 3 min).
  • NO2, NO3 and NH4, on Technicon or Alliance analysis lines coupled to filter ramps (acquisition frequency: 4min).
  • Photosynthetic parameters: variable fluorescence at MAP.

An automatic sampler can also be coupled to the culture system to perform a series of samples for further analysis.

Other parameters of interest are measured either directly in the laboratory or using other LOV platforms. The measurements usually performed are for particulate carbon and nitrogen (at CHN); dissolved carbon (DIC); cellular constituents : lipids (indicative measurements after Nile Red tagging and biochemical measurements after extraction), proteins and sugars (intracellular or excreted); pigment procession (by HPLC) or chlorophyll alone (by fluorescence or spectrophotometry); nitrogenase (GC) activity monitored online in a dedicated incubator; cell cycle (flow cytometer after DNA tagging). It is also possible to perform optical measurements by spectrophotometry and spectrofluorimetry.

 

 

Perspectives

In the future, further improvements will be made to the PhytoPULSE, in particular by automating new sensors or measuring instruments. In particular, it is planned to set up a continuous measurement of dissolved oxygen and, in the longer term, a regulation of this oxygen level. The use of the Odin software (INRIA) for system monitoring and control should be generalized.

 

Contact : Amélie Talec - Tél: 04 93 76 38 52 - Amelie.Talec -at- obs-vlfr.fr

Scientific Production Associated to the PHYTOPULSE

Bonnefond, H., Lie, Y., Pruvost, E., Talec, A., Bernard, O., & Sciandra, A. (2019). Improvement of microalgae strains by dynamic darwinian selection pressure. Paper presented at the Alg’in Provence, Arles, France.
Bernard, O., Bonnefond, H., Sciandra, A., Pruvost, É., & Grimaud, G. (2019). United States Patent No.
Demory, D., Combe, C., Hartmann, P., Talec, A., Pruvost, E., Hamouda, R., Souillé, F., Lamare, P.-O., Bristeau, M.-O., Sainte-Marie, J., Rabouille, S., Mairet, F., Sciandra, A., & Bernard, O. (2018). How do microalgae perceive light in a high-rate pond? Towards more realistic Lagrangian experiments. Royal Society Open Science, 5(5). doi:10.1098/rsos.180523
Bonnefond, H., Lie, Y., Pruvost, E., Talec, A., Bernard, O., & Sciandra, A. (2017). Evolution engineering for enhancing photosynthetic efficiency of high density cultures of Tisochrysis lutea. Paper presented at the 6th congress of the International Society for Applied Phycology, Nantes, France.
Bonnefond, H., Grimaud, G., Rumin, J., Bougaran, G., Talec, A., Gachelin, M., Boutoute, M., Pruvost, E., Bernard, O., & Sciandra, A. (2017). Continuous selection pressure to improve temperature acclimation of Tisochrysis lutea. Plos One, 12(9), e0183547. doi:10.1371/journal.pone.0183547
Bonnefond, H., Talec, A., Pruvost, E., Boutoute, M., Grimaud, G. M., Lie, Y., Sauvée, T., Sciandra, A., & Bernard, O. (2017). Potential of evolution engineering for enhancing productivity of microalgae. Paper presented at the AlgaEurope 2017 Conference, Berlin.
Bonnefond, H., Moelants, N., Talec, A., Mayzaud, P., Bernard, O., & Sciandra, A. (2017). Coupling and uncoupling of triglyceride and beta-carotene production by Dunaliella salina under nitrogen limitation and starvation. Biotechnology for Biofuels, 10(1), 25. doi:10.1186/s13068-017-0713-4
Bonnefond, H., Moelants, N., Talec, A., Bernard, O., & Sciandra, A. (2016). Concomitant effects of light and temperature diel variations on the growth rate and lipid production of Dunaliella salina. Algal Research, 14, 72-78. doi:10.1016/j.algal.2015.12.018
Bonnefond, H., Bernard, O., Pruvost, E., & Sciandra, A. (2016). France Patent No.
Rabouille, S., Talec, A., Bernard, O., & Sciandra, A. (2015). Nitrogen and carbon fluxes in unicellular, diazotrophic cyanobacteria: from cultures to models. Paper presented at the ASLO Aquatic Sciences Meeting, Granada, Spain.
Combe, C., Hartmann, P., Bernard, O., Rabouille, S., Talec, A., Pruvost, E., & Sciandra, A. (2015). Long-term adaptative response to high-frequency light signals in the unicellular photosynthetic eukaryote Dunaliella salina. Paper presented at the ASLO Aquatic Sciences Meeting, Granada, Spain.
Combe, C., Hartmann, P., Rabouille, S., Talec, A., Bernard, O., & Sciandra, A. (2015). Long-term adaptive response to high-frequency light signals in the unicellular photosynthetic eukaryote Dunaliella salina. Biotechnology And Bioengineering, 112(6), 1111-1121. doi:10.1002/bit.25526
Bonnefond, H., Pruvost, E., Sciandra, A., & Bernard, O. (2015). Continuous selection pressure to enlarge temperature niche of Tisochrysis lutea. Paper presented at the 6th European Phycological Congress, London. https://www.tandfonline.com/doi/full/10.1080/09670262.2015.1069493
Rumin, J., Bonnefond, H., Saint-Jean, B., Rouxel, C., Sciandra, A., Bernard, O., Cadoret, J.-P., & Bougaran, G. (2015). The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae. Biotechnology for Biofuels, 8. doi:10.1186/s13068-015-0220-4
Mairet, F., Bernard, O., Pruvost, E., & Sciandra, A. (2015). France Patent No.
Grimaud, G. M., Rabouille, S., Dron, A., Sciandra, A., & Bernard, O. (2014). Modelling the dynamics of carbon–nitrogen metabolism in the unicellular diazotrophic cyanobacterium Crocosphaera watsonii WH8501, under variable light regimes. Ecological Modelling, 291(0), 121-133. doi:10.1016/j.ecolmodel.2014.07.016
Mocquet, C., Sciandra, A., Talec, A., & Bernard, O. (2013). Cell cycle implication on nitrogen acquisition and synchronization in Thalassiosira weissflogii (Bacyllariophyceae). Journal of Phycology, 49, 371–380. doi:10.1111/jpy.12045
Grimaud, G. M., Dron, A., Rabouille, S., Sciandra, A., & Bernard, O. (2013). Modelling light-dark regime influence on the carbon-nitrogen metabolism in a unicellular diazotrophic cyanobacterium. Paper presented at the Proceedings of the 12th IFAC Symposium on Computer Applications in Biotechnology, Mumbai, India. doi:10.3182/20131216-3-IN-2044.00025
Dron, A., Rabouille, S., Claquin, P., Talec, A., Raimbault, V., & Sciandra, A. (2013). Photoperiod length paces the temporal orchestration of cell cycle and carbon-nitrogen metabolism in Crocosphaera watsonii. Environmental Microbiology, 15(12), 3292-3304. doi:10.1111/1462-2920.12163
Talec, A., Philistin, M., Ferey, F., Walenta, G., Irisson, J. O., Bernard, O., & Sciandra, A. (2013). Effect of gaseous cement industry effluents on four species of microalgae. Bioresour Technol, 143, 353-359. doi:10.1016/j.biortech.2013.05.104
Lacour, T., Sciandra, A., Talec, A., Mayzaud, P., & Bernard, O. (2012a). Neutral Lipid and Carbohydrate Productivities as a Response to Nitrogen Status in Isochrysis Sp. (T-Iso; Haptophyceae): Starvation Versus Limitation1. Journal of Phycology, 48(3), 647-656. doi:10.1111/j.1529-8817.2012.01154.x
Lacour, T., Sciandra, A., Talec, A., Mayzaud, P., & Bernard, O. (2012b). Diel variations of carbohydrates and neutral lipids in nitrogen-sufficient and nitrogen-starved cyclostat cultures of Isocgrysis sp. Journal of Phycology, 48(4), 966-975. doi:10.1111/j.1529-8817.2012.01177.x
Dron, A., Rabouille, S., Claquin, P., & Sciandra, A. (2012). Photoperiod length shapes the carbon-nitrogen metabolism in Crocosphera watsonii. Paper presented at the 14th International Symposium on Phototrophic Prokaryotes, Porto, Portugal.
Müller, M. N., Beaufort, L., Bernard, O., Pedrotti, M. L., Talec, A., & Sciandra, A. (2012). Influence of CO2 and nitrogen limitation on the coccolith volume of Emiliania huxleyi (Haptophyta). Biogeosciences, 9(10), 4155-4167. doi:10.5194/bg-9-4155-2012
Dron, A., Rabouille, S., Claquin, P., Le Roy, B., Talec, A., & Sciandra, A. (2012). Light–dark (12:12) cycle of carbon and nitrogen metabolism in Crocosphaera watsonii WH8501: relation to the cell cycle. Environmental Microbiology, 14(4), 967-981. doi:10.1111/j.1462-2920.2011.02675.x
Dron, A., Rabouille, S., Claquin, P., Chang, P., Raimbault, V., Talec, A., & Sciandra, A. (2012). Light:dark (12:12 h) quantification of carbohydrate fluxes in Crocosphaera watsonii. Aquatic Microbial Ecology, 68(1), 43-55. doi:Doi 10.3354/Ame01600
Mairet, F., Bernard, O., Masci, P., Lacour, T., & Sciandra, A. (2011). Modelling neutral lipid production by the microalga Isochrysis aff. galbana under nitrogen limitation. Bioresour Technol, 102(1), 142-149. doi:10.1016/j.biortech.2010.06.138
Mairet, F., Bernard, O., Lacour, T., & Sciandra, A. (2011). Modelling microalgae growth in nitrogen limited photobiorector for estimating biomass, carbohydrate and neutral lipid productivities. Paper presented at the 18th IFAC World Congress, Milan, Italy. doi:10.3182/20110828-6-IT-1002.03165
Müller, M. N., Bernard, O., & Sciandra, A. (2011). Influence of nutrient limitation and CO2 on the cell and coccolith volume of coccolithophores: Implications for the sediment record? Paper presented at the Symposium: Ocean acidification and implications for living marine resources in the Southern Hemisphere, Camberra, Australia.
Mairet, F., Bernard, O., Masci, P., Lacour, T., & Sciandra, A. (2010a). Modelling neutral lipid productivity in photobioreactors under nitrogen deprivency. Paper presented at the 8th European Workshop on Biotechnology of Microalgae, Nuthetal, Germany.
Mairet, F., Bernard, O., Masci, P., Lacour, T., & Sciandra, A. (2010b). Modelling neutral lipid production in microalgae. Paper presented at the 2010 IFAC International Symposium on Dynamics and Control of Process Systems (DYCOPS), Leuven, Belgium. Retrieved from https://ifac.papercept.net/conferences/scripts/abstract.pl?ConfID=18&Number=90
Mocquet, C., Bernard, O., & Sciandra, A. (2010). Cell cycle modeling for microalgae grown under a light/dark cycles. Paper presented at the 11th IFAC Symposium on Computer Applications in Biotechnology, Leuven, Belgium. doi:10.3182/20100707-3-BE-2012.0072
MacIntyre, H., Babin, M., Gelay, A., Goldman, E., Huot, Y., Sciandra, A., & Sherman, T. (2009). Photosynthetic Response of the Toxic Raphidophyte Heterosigma Akashiwo during the Imposition of, and Recovery from, Light- and Nutrient Stress. Paper presented at the Journal of Phycology. <Go to ISI>://WOS:000263910300030
MacIntyre, H. L., Babin, M., Gelay, A., Goldman, E., Huot, Y., Sherman, T., & Sciandra, A. (2009). The effect of nitrogen starvation and resupply on photosynthesis and growth in the toxic raphidophyte Heterosigma akashiwo. Paper presented at the ASLO Aquatic Sciences Meeting, Nice, France.
Esposito, S., Bernard, O., Sciandra, A., Botte, V., Ludicone, D., & Ribera d' Alcala, M. (2009). From the ocean to the photobioreactors: study of the impact on productivity of phytoplankton photoresponses to light variation. Paper presented at the ASLO Aquatic Sciences Meeting, Nice, France.
Mocquet, C., Sciandra, A., Bernard, O., Esposito, S., & Gelay, A. (2009). Effect of cell cycle on nitrate uptake of the marine diatom Thalassiosira weissflogii grown in a cyclostat. Implication for modeling global fluxes. Paper presented at the ASLO Aquatic Sciences Meeting, Nice, France.
Bernard, O., & Sciandra, A. (2009). A new photobioreactor model dealing with nitrogen limitation. Paper presented at the ASLO Aquatic Sciences Meeting, Nice, France.
Lacour, T., Mayzaud, P., Bernard, O., Gelay, A., & Sciandra, A. (2009). Enhancing lipid production by phytoplankton cells: nitrate depletion and light/dark cycle. Paper presented at the ASLO Aquatic Sciences Meeting, Nice, France.
Bernard, O., Masci, P., & Sciandra, A. (2009). A photobioreactor model in nitrogen limited conditions. Paper presented at the Mathmod 09 conference, Vienna, Ostrich.
Lacour, T., Gelay, A., Mayzaud, P., & Sciandra, A. (2008). Lipid composition of Isochrysis galbana grown in a light/dark cycle and nitrate depletion: a lipid acclimation to environmental changes. Paper presented at the 18th International Symposium on Plant Lipids (ISPL), Bordeaux.
Bernard, O., Sciandra, A., & Madani, S. (2008). Multimodel analysis of the response of the coccolithophore Emiliania huxleyi to an elevation of pCO2 under nitrate limitation. Ecological Modelling, 211(3-4), 324-338. doi:10.1016/j.ecolmodel.2007.09.027
Fani, F., Babin, M., Gelay, A., Hout, Y., Lazzara, L., MacIntyre, H., & Sciandra, A. (2007). Variability in the photophysiology of the harmful alga Heterosigma akashiwo (Raphidophyceae). Paper presented at the Congresso SItE-AIOL, Ancona.
Bernard, O., Sciandra, A., & Sialve, B. (2007). Toward modeling of lipids synthesis in marine phytoplanktonic microalgae under nitrogen starvation. Paper presented at the 7th European Workshop of the European society of Microalgal Biotechnology, Potsdam.
Bernard, O., Madani, S., & Sciandra, A. (2006). Modelling the response of the marine microalgae Emiliania huxleyi to an elevation of pCO2. Paper presented at the Mathmod 06 conference, Vienna, Austria.
Kasalicky, V., Zrotalova, K., Sciandra, A., Babin, M., & Prasil, O. (2006). Photoacclimation of the marine diatom Thalassiosira pseudonana to natural fluctuating light and constant conditions under nitrogen deficiency. Paper presented at the XV FESPB Congress, Lyon, France.
Manté, C., Nérini, D., & Sciandra, A. (2006). Combien de temps vit le dinoflagellé Prorocentrum minimum? Une approche via la statistique des processus. Paper presented at the XXIIème Séminaire de la Société Francophone de Biologie Théorique - Caractérisation et analyse de l’hétérogénéité dans les systèmes biologiques., Saint-Flour, France.
Manté, C., Nerini, D., Sciandra, A., & Yao, A. F. (2006). Estimating the Hurst exponent of self-similar processes. Application in marine ecology. Paper presented at the COMPSTAT 2006 - 17th conference of IASC-ERS, Rome, Italy.
Baklouti, M., Faure, V., Pawlowski, L., & Sciandra, A. (2006). Investigation and sensitivity analysis of a mechanistic phytoplankton model implemented in a new modular numerical tool (Eco3M) dedicated to biogeochemical modelling. Progress In Oceanography, 71(1), 34-58. doi:10.1016/j.pocean.2006.05.003
Sciandra, A. (2004). Nitrate uptake in simulated deep mixed-layer. Interference with photosynthesis and cell division , MAG Meeting. Paper presented at the MAG Meeting, Villefranche sur Mer.
Sciandra, A., Harlay, J., Lefèvre, D., Lemée, R., Rimmelin, P., Denis, M., & Gattuso, J.-P. (2004). Response of the coccolithophorid Emiliania huxleyi to elevated pCO2 under nitrate limitation. Paper presented at the The Ocean in a High CO2 World, UNESCO, Paris, France.
Pawlowski, L., Sciandra, A., & Bernard, O. (2004). Interactive effects of light and cell division on the nitrate uptake of Thalassiosira weissflogii grown in an experimentally simulated deep mixed layer. Paper presented at the EGU, Nice, France.
Denis, M., Sciandra, A., Harlay, J., Lefèvre, D., Lemée, R., Rimmelin, P., & Gattuso, J.-P. (2004). Effect of elevated pCO2 on optical properties of the coccolithophorid Emiliania huxleyi grown under nitrate limitation. Paper presented at the ISAC XXII International Congress, Montpellier, France.
Denis, M., Sciandra, A., Harlay, J., Lefevre, D., Lemee, R., Rimmelin, P., & Gattuso, J. P. (2004). Effect of elevated PCO2 on optical properties of the coccolithophorid Emiliania huxleyi grown under nitrate limitation. Cytometry Part A, 59A(1), 105-105. Retrieved from <Go to ISI>://WOS:000221319900236
Sciandra, A., Harlay, J., Lefevre, D., Lemee, R., Rimmelin, P., Denis, M., & Gattuso, J. P. (2003). Response of coccolithophorid Emiliania huxleyi to elevated partial pressure of CO2 under nitrogen limitation. Marine Ecology Progress Series, 261, 111-122. doi:10.3354/meps261111
Sciandra, A., Harlay, J., Lefèvre, D., Lemée, R., Rimmelin, P., Denis, M., & Gattuso, J.-P. (2003). Response of the coccolithophorid Emiliania huxleyi to elevated pCO 2 under nitrate limitation. Paper presented at the EGS-AGU-EUG Joint Assembly, Nice, France.
Denis, M., Sciandra, A., Harlay, J., Lefèvre, D., Lemée, R., Rimmelin, P., & Gattuso, J.-P. (2003). Impact of elevated pCO2 on optical properties of the coccolithophorid Emiliania huxleyi grown under nitrate limitation. Paper presented at the EGS-AGU-EUG Joint Assembly, Nice, France.
Bourguet, N., Goutx, M., Van Wambeke, F., Guigue, C., Sciandra, A., & Gattuso, J.-P. (2003). Effects of increased CO2 partial pressure on the chemical composition and microbial degradation of the microalgae Emiliania huxleyi. Paper presented at the EGS-AGU-EUG Joint Assembly, Nice, France.
Pawlowski, L., Bernard, O., Le Floc'h, E., & Sciandra, A. (2002a). Le chémostat: un outil de développement et de validation de modèles de croissance phytoplanctonique. Paper presented at the XXIIème Séminaire de la Société Francophone de Biologie Théorique - Ecologie Aquatique : Théorie et Expérience, Saint-Flour.
Sciandra, A. (2002). Le chémostat: Théorie et retour au réel. Paper presented at the XXIIème Séminaire de la Société Francophone de Biologie Théorique - Ecologie Aquatique : Théorie et Expérience, Saint-Flour.
Arino, J., Gouze, J. L., & Sciandra, A. (2002). A discrete, size-structured model of phytoplankton growth in the chemostat: introduction of inhomogeneous cell division size. J Math Biol, 45(4), 313-336. doi:10.1007/s002850200160
Pawlowski, L., Bernard, O., Le Floc'h, E., & Sciandra, A. (2002b). Non linear modelling of the coupling between carbon and nitrogen pathways during phytoplankton growth. Validation with chemostat experiments. Paper presented at the ASLO meeting, Hawaii.
Pawlowski, L., Bernard, O., Le Floc'h, E., & Sciandra, A. (2002c). Qualitative behaviour of phytoplankton growth model in photobioreactor. Paper presented at the Proceedings of the 15th IFAC World Congress, Barcelona, Spain. doi:10.3182/20020721-6-ES-1901.01382
Stramski, D., Sciandra, A., & Claustre, H. (2002). Effects of temperature, nitrogen, and light limitation on the optical properties of the marine diatom Thalassiosira pseudonana. Limnology and Oceanography, 47(2), 392-403. doi:10.4319/lo.2002.47.2.0392
Le Floc'h, E., Malara, G., & Sciandra, A. (2002). An automatic device for in vivo absorption spectra acquisition and chlorophyll estimation in phytoplankton cultures. Journal of Applied Phycology, 14(6), 435-444. doi:10.1023/A:1022338930747
Bernard, O., & Sciandra, A. (2001). Software sensor as a new tool to monitor the internal nitrogen quota of algae. Paper presented at the ASLO meeting, Albuquerque, U.S.
Le Floc'h, E., & Sciandra, A. (2001). Adaptation of pigment composition to interactive effects of light an nitrogen in Rhodomonas salina (cryptophyceae). Paper presented at the ASLO meeting, Abulquerque.
Bruyant, F., Babin, M., Sciandra, A., Marie, D., Genty, B., Claustre, H., Blanchot, J., Bricaud, A., Rippka, R., Boulben, S., Louis, F., & Partensky, F. (2001). An axenic cyclostat of Prochlorococcus PCC 9511 with a simulator of natural light regimes. Journal of Applied Phycology, 13(2), 135-142. doi:10.1023/A:1011144310988
Bernard, O., Sciandra, A., & Sallet, G. (2001). A non-linear software sensor to monitor the internal nitrogen quota of phytoplanktonic cells. Oceanologica Acta, 24(5), 435-442. doi:10.1016/S0399-1784(01)01155-0
Sciandra, A., Lazzara, L., Claustre, H., & Babin, M. (2000). Responses of the growth rate, pigment composition and optical properties of Cryptomonas sp. to light and nitrogen stresses. Marine Ecology Progress Series, 201, 107-120. doi:10.3354/meps201107
Arino, J., Gouzé, J. L., & Sciandra, A. (2000). A discrete, size-structured model of phytoplankton growth in the chemostat. In Rapport N°: 3963, Thème 4 - Simulation et optimisation de systèmes complexes (Projet COMORE) (pp. 24): INRIA.
Bernard, O., Sallet, G., & Sciandra, A. (1999). Use of non linear software sensors to monitor the internal state of a culture of microalgae. Paper presented at the 14th IFAC World Congress, Beijing, China. doi:10.1016/S1474-6670(17)56991-8
Collos, J. Y., Mornet, F., Sciandra, A., Waser, N., Larson, A., & Harrisson, P. J. (1999). An optical method for the rapid measurement of micromolar concentrations of nitrate in marine phytoplankton cultures. Journal of Applied Phycology, 11(2), 189-194. doi:10.1023/A:1008046023487
Lazzara, L., Sciandra, A., Claustre, C., Babin, M., & Mori, G. (1999). Growth rate, absorption and fluorescence, elemental and pigment composition of Cryptomonas sp. under light and nitrogen stresses. Paper presented at the 2nd European Phycologycal Congress, Montecatini Terme, Italy.
Leboulanger, C., Martin-Jézéquel, V., Descolas-gros, C., Sciandra, A., & Jupin, H. (1998). Photorespiration in continuous cultures of Dunaliella teriolecta (Chlorophyta) : relationships between serine, glycine and extracellular glycolate. Journal of Phycology, 34(4), 651-654. doi:10.1046/j.1529-8817.1998.340651.x
Bernard, O., Sallet, G., & Sciandra, A. (1998). Nonlinear observers for a class of biological systems: Application to validation of a phytoplanktonic growth model. IEEE - Transactions on Automatic Control, 43(8), 1056-1065. doi:10.1109/9.704977
Bernard, O., Sciandra, A., & Sallet, G. (1998). High gain nonlinear softwares to monitor a culture of microalgae. Paper presented at the Biodecision 98, Montpellier, France.
Collos, Y., Sciandra, A., Martin-Jézéquel, V., Slawick, G., & Garcia, N. (1998). Dilution of 15N in Dunaliella tertiolecta by uptake of an unidentified nitrogen compound following nitrate exhaustion. Comptes Rendus de l'Académie des Sciences - Series III - Sciences de la Vie, 321(8), 673-677. doi:10.1016/S0764-4469(98)80007-3
Sciandra, A., Gostan, J., Collos, Y., Descolas-Gros, C., Leboulanger, C., Martin-Jézéquel, V., Denis, M., Lefèvre, D., Copin-Montégut, C., & Avril, B. (1997). Growth compensating phenomena in continuous cultures of Dunaliella tertiolecta limited simultaneously by light and nitrate. Limnology and Oceanography, 42(6), 1325-1339. doi:10.4319/lo.1997.42.6.1325
Sciandra, A. (1997). Cycle cellulaire et fluctuations de populations phytoplanctoniques en interaction avec une ressource variable. Conditions d'application des modèles à quota. Paper presented at the Rapport JGOFS-France.
Bernard, O., Malara, G., & Sciandra, A. (1996). The effects of a controlled fluctuating nutrient environment on continuous cultures of phytoplankton monitored by a computer. Journal of Experimental Marine Biology and Ecology, 197(2), 263-278. doi:10.1016/0022-0981(95)00161-1
Descolas-Gros, C., Denis, M., Gostan, J., Jupin, H., Collos, Y., Leboulanger, C., Lefèvre, D., Martin-Jézéquel, V., & Sciandra, A. (1996). Carboxylation and nutrient uptake. Paper presented at the Integrated Marine System Analysis, Brugge.
Sciandra, A. (1996). Growth compensating phenomena in continuous cultures of Dunaliella tertiolecta limited simultaneously by light and nitrate. Paper presented at the ASLO meeting, San Diego, USA.
Sciandra, A. (1995). Etude de la croissance et de l'absorption de nitrate chez une algue phytoplanctonique (Prorocentrum minimum: dinophyceae) soumises à des apports impulsionnels et périodiques de nitrate. In A. Pavé & J. L. Gouze (Eds.), Les Cahiers d'EDORA II (Vol. N°: 2530, pp. 71-78): INRIA.
Sciandra, A., & Ramani, P. (1994). The steady states of continuous cultures with low rates of medium renewal per cell. Journal of Experimental Marine Biology and Ecology, 178(1), 1-15. doi:10.1016/0022-0981(94)90221-6
Sciandra, A., & Amara, R. (1994). Effects of nitrogen limitation on growth and nitrite excretion rates of the dinoflagellate Prorocentrum minimum. Marine Ecology Progress Series, 105(3), 301-309. doi:10.3354/meps105301
Sciandra, A. (1994). Effects of nitrogen limitation on growth and nitrite excretion rates of the dinoflagellate Prorocentrum minimum. Paper presented at the Proc. First MEDIPELAGOS Modeling Workshop, Blanes, Spain.
Sciandra, A. (1993). Dynamics of nitrate uptake and cell division rates of the dinoflagellate Prorocentrum minimum in a nitrate pulsed experiment. Paper presented at the 3rd International Colloquium of The Oceanographic Society, Seattle, USA.
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LOV - 06/11/19